Objective: To study the effects of different drying conditions and treatments on the content of active ingredients in fresh roots of Bupleurum chinense so as to provide basis for determining the best drying method and improving the quality of Bupleurum chinense. Methods: Radix Bupleuri samples were dried by five methods: sun drying, shade drying, 50 C drying, 100 C drying and microwave drying.
The content of ethanol-soluble extract was determined by hot leaching method, saikosaponin a and D in Bupleurum chinense were determined by HPLC, total saikosaponin in Bupleurum chinense was determined by spectrophotometry, and the content of effective components in Bupleurum chinense was comprehensively evaluated by subordinate function analysis under different drying methods.
Saikosaponin C is extracted from the root of Bupleurum chinense DC. It efficiently inhibited LPS-induced apoptotic cell death via inhibition of caspase-3 activation and caspase-3-mediated-FAK degradation. It exhibits anti-HBV activity. Saikosaponin C
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