ABSTRACT: OBJECTIVE: To investigate the effect of microwave drying equipment on the content of Codonopsis alkynoside in Codonopsis pilosula so as to provide basis for scientific evaluation and effective control of its quality. METHODS: HPLC, gradient elution and ultrasonic extraction were used. The chromatographic conditions were as follows: Kromasil C18 column (4.6 mm *250 mm, 5 micron), flow rate: 1.0 mL/min, detection wavelength: 267 nm, column temperature: 25 C, mobile phase: acetonitrile-0.05% phosphoric acid gradient elution system.
RESULTS: The content of Codonopsis glycoside was higher in different microwave time (5 min, 5.5 min, 6 min, medium fire) and 25% honey-baked Codonopsis pilosula microwave for 5 min under the same conditions.
CONCLUSION: Different microwave time can affect the content of Codonopsis alkynoside in Codonopsis pilosula. The content of Codonopsis alkynoside in Codonopsis pilosula is higher when it is roasted with 25% honey and microwave for 5 minutes. Meanwhile, the determination of Codonopsis alkynoside in Codonopsis pilosula by HPLC is simple, reliable and reproducible. It can be used as an effective method for quality control of Gansu genuine medicinal material Codonopsis
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